Regulatory

Part:BBa_K3007001:Design

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-14)


Uric acid-responsive promoter (PhucR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007001

When we used the part, the sequence at the 5' end of this part in the lac promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained.

It is a promoter modified from lac promoter. As at the 3' end of lac promoter, there is a RBS sequence RBS0029 (tcacacaggaaa). As a result, no additional RBS is needed.

Source

The key component hucO comes from Deinococcus radiodurans.

References